ISOLATION, IDENTIFICATION AND USE OF STRAINS OF BACTERIA OF THE GENUS BACILLUS IN A MICROBIOLOGICAL TEST FOR THE DETERMINATION OF ANTIBIOTICS IN MILK

The use of antibiotics in the treatment livestock has increased productivity meat and dairy industry. flip side effectiveness drugs this group is presence effects, which have caused concern about widespread cause need to control residual content antibacterial agents products. From soil samples collected 7 regions Kazakhstan, 14 microbial isolates were isolated identified as B. licheniformis, sonorensis, cereus, simplex, thuringiensis, pumilis, mojavensis, subtilis, atrophaeus, paralicheniformis. Sensitivity testing strains showed that susceptible 13 belonging lincosamide, ansamycin, quinolone, macrolide, fluoroquinolone, tetracycline, nitrobenzene, aminoglycoside, glycopeptide beta-lactam antibiotics. proteolytic strain Bacillus licheniformis T7 seems promising a test culture. It highly sensitive antibiotics, grows rapidly on variety nutrient media, alkaline spore-forming can be cultured at 37-55°C. peculiarity change pH medium from 5.0 7.0 above makes it possible bromcresol purple growth-detecting dye. This been shown experiments using antibiotic milk LB agar broth 5.0. In absence growth-inhibiting culture vigorously, causes shift 7.99 color bromocresol yellow purple. results promise for used bacterial development microbiological detection milk.